Histone-mediated transduction as an efficient means for gene delivery
Study by Dr. Kylie Wagstaff
Promo Code: Flurmie
https://pubmed.ncbi.nlm.nih.gov/17327830/
Abstract
Gene delivery into the nucleus of eukaryotic cells is inefficient, largely because of the significant barriers within the target cell of the plasma membrane and nuclear envelope. Recently, a group of basic proteins, including the HIV-1 Tat protein and the four core histones, have been shown to enter cells through a novel energy- and receptor-independent manner. Here, we show that engineered histone H2B proteins are able to mediate the efficient delivery of either green fluorescent protein or DNA into HeLa cells through the process of "Histone-Mediated Transduction" (HMT), with further enhancement achieved by utilizing a dimer of histones H2B and H2A. Subsequent nuclear delivery was accelerated approximately two-fold by the addition of an optimized nuclear localization signal to histone H2B, thereby increasing the affinity of interaction with components of the cellular nuclear import machinery, resulting in increased expression of a reporter gene. Further, we demonstrate that the domains responsible for this histone transduction are located in the N-terminal tail and globular regions of histone H2B. HMT represents a new, efficient, and technically non-demanding means to deliver DNA to the nucleus of intact cells, including embryonic stem cells, which has important applications in gene therapy and cancer therapeutics.
Just posting about some science and stuff.
Learning about gene delivery. Having some food.
I am now going to post a bewildering array of posts previously done.
Just looking for a pattern.
Dr. Kylie in Aussie.
related:
related:
Update: I am now in the Interrogation Room.
Eating pizza and discussing gene therapy.
I didn’t get very much sleep last night.
Sometimes, manic.
Our products are only available at Amazon, and Printify.
+1 Statins Good.
Two years ago. Here.
hint: Ute strongly suggests it’s the gene editing shots.
A little hair of the dog that bit you!
CJB dropped another jaw-dropping summary of the original Day Tapes
https://odysee.com/@CJBbooks.com:8/Divine-Mission-to-Destroy-Us-A-Master-Class-on-the-Exodus-Formula-Demolishing-America-and-the-World:a
"Gene delivery into the nucleus of eukaryotic cells is inefficient, largely because of the SIGNIFICANT BARRIERS within the target cell of the plasma membrane and nuclear envelope."
Here we go again. The Greeks against the Walls of Troy. Only trickery will work. Achilles is still destined to die during the invasion.
These fancy lab experiments are very cute. But when do we get to see people transforming into orangutans?